Skip to Content
MilliporeSigma
All Photos(1)

Documents

819532

TSKgel® Amide-80 HPLC Column

phase carbamoyl, L × I.D. 5 cm × 4.6 mm, 5 μm particle size

Synonym(s):

TSKgel® Amide-80, 5 cm x 4.6 mm ID, 5 μm

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41115711
eCl@ss:
32110501

material

stainless steel hardware

agency

suitable for USP L68

product line

TSKgel®

manufacturer/tradename

Tosoh 19532

parameter

0-100% organic solvent
10-80 °C temperature
150 bar max. pressure

technique(s)

HPLC: suitable

L × I.D.

5 cm × 4.6 mm

surface area

450 m2/g

matrix

silica particle platform

matrix active group

carbamoyl phase

particle size

5 μm

pore size

100 Å

operating pH

2.0-7.5

separation technique

hydrophilic interaction (HILIC)

Looking for similar products? Visit Product Comparison Guide

General description

TSKgel Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, the new, high efficiency, 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service