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82021-U

Hamilton® PRP-1 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.1 mm, stainless steel hardware

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

Hamilton PRP-1 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.1 mm, stainless steel hardware

material

stainless steel column

agency

suitable for USP L21

manufacturer/tradename

Hamilton

packaging

1 ea of

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.1 mm

matrix

PS-DVB particle platform
polymeric

matrix active group

polymer

particle size

5 μm

pore size

100 Å

operating pH

1-14

separation technique

reversed phase

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General description

PRP-1 is a co-polymer of poly (styrene-divinylbenzene). Its surface is chemically neither homogenous nor stable during use. Hamilton PRP-1 HPLC Column, is used for large scale purification of oligonucleotides, generally used in NMR studies.
The Hamilton PRP-1, polymeric reversed phase columns are Ideal for high pH (pH 8-13) operation or with analytes that give poor chromatogrpahy on silica-based columns.

Application

Hamilton PRP-1 HPLC Column was used in experimental studies employing liquid chromatography (LC); with reversed phase separation (RPLC) using only pressurized hot water (PHW) as a mobile phase. It also is suitable for being used in large-scale purification of synthetic oligonucleotides, with a phosphate/methanol/acetonitrile solvent system.

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Characterization of some commercial poly (styrene-divinylbenzene) copolymers for reversed-phase HPLC1.
Stuurman HW
Chromatographia, 23 (5), 341-349 (1987)
Large-scale purification of synthetic oligonucleotides and carcinogen-modified oligodeoxynucleotides on a reverse-phase polystyrene (PRP-1) column.
Guanjin H and Krugh TR.
Analytical Biochemistry, 190 (1), 21-25 (1990)
Liquid chromatography at elevated temperatures with pure water as the mobile phase.
Kari H and Riekkola ML.
Trends in Analytical Chemistry, 27 (1), 1-14 (2008)

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