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TSKgel® Amide-80 HPLC Column

phase carbamoyl, L × I.D. 10 cm × 3 mm, 3 μm particle size

Synonym(s):

TSKgel® Amide-80, 10 cm x 3 mm ID, 3 μm

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About This Item

UNSPSC Code:
41115711

material

stainless steel

agency

suitable for USP L68

product line

TSKgel®

manufacturer/tradename

Tosoh 22851

parameter

0-100% organic solvent
10-50 °C temperature
200 bar max. pressure

technique(s)

HPLC: suitable

L × I.D.

10 cm × 3 mm

surface area

450 m2/g

matrix

spherical silica

matrix active group

carbamoyl phase

particle size

3 μm

pore size

100 Å

operating pH

2.0-7.5

separation technique

hydrophilic interaction (HILIC)

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General description

TSKgel® Amide-80 columns are packed with 3, 5, or 10 micron spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. Target applications include the analysis of saccharides, glycans, oligosaccarides, peptides and polar compounds from natural products. Unique advantages of Amide-80 phase for saccharide analysis include novel hydrogen bonding retention mechanism between hydroxyl groups of the sample and the carbamoyl group in the stationary phase. The stationary phase does not react with reducing sugars and can be used at elevated temperatures (4 to 80 °C) to prevent peak splitting of carbohydrates that can occur at lower temperatures. In addition, high efficiency 3 micron columns offer shorter analysis time and enhanced sensitivity for LC-MS applications.

Physical form

Shipped in 85% acetonitrile - 15% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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