G001505A
HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C18
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)
mobile phase: [A] (0.1% TFA in water):(0.1% TFA in acetonitrile) 95:5; [B] (0.1% TFA in water):(0.1% TFA in acetonitrile):50:50;
gradient: 0-100% B in 65 min
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 215 nm
injection: 50 μL
sample: carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate
suitability
application for HPLC
application(s)
life science and biopharma
Legal Information
analytical column
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