G001512
HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #1)
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Discovery BIO Wide Pore C18, 15cm × 4.6mm I.D., 5μm particles (568222-U)
mobile phase: [A] (80:20), 0.1% TFA in water:0.1% TFA in acetonitrile; [B] (66:34), 0.1% TFA in water:0.1% TFA in acetonitrile
gradient: 0-100% B in 14 min after 1 minute delay
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 220 nm
injection: 10 μL
sample: Sigma Peptide Mix (Cat. No. P2693)
suitability
application for HPLC
application(s)
life science and biopharma
Legal Information
analytical column
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