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G005379

HPLC Analysis of Basic Peptides (Angiotensin Analogs) on BIOshell A160 Peptide C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: BIOshell A160 Peptide C18, 10 cm x 3 mm I.D., 2.7 μm particles (66908-U)
mobile phase: [A] 0.1% (v/v) formic acid, pH 4.0 (titrated with ammonium hydroxide); [B] 50:50, (0.175% formic acid in water, pH 4.0) : acetonitrile
gradient: 20 to 50% B in 15 min
flow rate: 0.6 mL/min
pressure: 3002 psi (207 bar)
column temp.: 35 °C
detector: UV, 215 nm
injection: 2.0 μL
sample: 0.5 g/L ea. peptide

suitability

application for HPLC

application(s)

life science and biopharma
pharmaceutical (small molecule)

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