Skip to Content
MilliporeSigma
All Photos(1)

Documents

G005593

HPLC Analysis of Flavonoids in Lime Juice on Ascentis® Express C18

application for HPLC, application for SPE

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: lime juice hand-squeezed in laboratory, centrifuged and filtered through a 0.45 μm nylon filter disc (Acrodisc)
column: Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 μm particles (53826-U)
mobile phase: [A] water/formic acid (99.9:0.1); [B] water/acetonitrile/isopropanol/formic acid (39.9:20:40:0.1)
gradient: 10% B for 3 min; 10 to 34% B in 30 min; 34 to 100% B in 1 min; held at 100% B for 6 min; 100 to10% B in 1 min
flow rate: 0.8 mL/min
column temp.: ambient
detector: PDA: 190–370 nm; chromatogram extracted at 283 nm
detector: ESI-MS: mass spectral range, 250-700 m/z; interval, 0.5 s; scan speed, 938 amu/s; nebulizing gas (N2) flow, 1.5 L/min; (interface temperature, 350 °C; heat block, 300 °C; desolvation line (DL) temperature, 300 °C; DL voltage, 34 V; probe voltage, +4.5 kV; Qarray voltage, 1.0 V; RF voltage, 90 V; detection gain, 1.05 kV)
injection: 2 μL

suitability

application for HPLC
application for SPE

application(s)

food and beverages

Analysis Note

Flavonoids are polyphenolic compounds ubiquitous in nature and classified, according to their chemical structure, into flavonols, flavones, flavanones, isoflavones, catechins, anthocyanidins and chalcones. They are widely present in fruits, vegetables, leaves, nuts, seeds, bark and beverages (tea, coffee, beer, wine and fruit drinks).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Eriocitrin
    Narirutin
    Hesperidin

analytical column

Product No.
Description
Pricing

mobile phase component

Product No.
Description
Pricing

standard

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service