G005688
LC/MS Analysis of Conjugated Bile Acids on Ascentis® Express C18
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 μm particles (53826-U)
mobile phase: [A] 10 mM ammonium acetate, pH 4 with acetic acid; [B] acetonitrile
gradient: 25 to 40% B in 10 min
flow rate: 1.0 mL/min (postcolumn split to MS)
column temp.: 35 °C
detector: MS, ESI(-), SIR
injection: 5 μL
sample: 10 mg/L each in mobile phase A
suitability
application for HPLC
application(s)
clinical
Legal Information
Analyte
Standard
analytical column
mobile phase component
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