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Click-Crosslinked Injectable Gelatin Hydrogels.

Advanced healthcare materials (2016-01-26)
Sandeep T Koshy, Rajiv M Desai, Pascal Joly, Jianyu Li, Rishi K Bagrodia, Sarah A Lewin, Neel S Joshi, David J Mooney
ABSTRACT

Injectable gelatin hydrogels formed with bioorthogonal click chemistry (ClickGel) are cell-responsive ECM mimics for in vitro and in vivo biomaterials applications. Gelatin polymers with pendant norbornene (GelN) or tetrazine (GelT) groups can quickly and spontaneously crosslink upon mixing, allowing for high viability of encapsulated cells, establishment of 3D elongated cell morphologies, and biodegradation when injected in vivo.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Alkyne functionalized gelatin, degree of substitution > 80%
Sigma-Aldrich
Azide functionalized gelatin, degree of substitution >80%