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  • Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases.

Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases.

Applied and environmental microbiology (2006-09-05)
Mi-Hwa Lee, Choong-Hwan Lee, Tae-Kwang Oh, Jae Kwang Song, Jung-Hoon Yoon
ABSTRACT

We cloned lipG, which encoded a lipolytic enzyme, from a Korean tidal flat metagenomic library. LipG was related to six putative lipases previously identified only in bacterial genome sequences. These enzymes comprise a new family. We partially characterized LipG, providing the first experimental data for a member of this family.

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2,3-Dimercapto-1-propanol tributyrate, 97%