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  • Reduction of inflammation and preservation of neurological function by anti-CD52 therapy in murine experimental autoimmune encephalomyelitis.

Reduction of inflammation and preservation of neurological function by anti-CD52 therapy in murine experimental autoimmune encephalomyelitis.

Journal of neuroimmunology (2015-07-23)
Michael J Turner, Petti T Pang, Nathalie Chretien, Evis Havari, Michael J LaMorte, Julian Oliver, Nilesh Pande, Elizabeth Masterjohn, Karen Carter, David Reczek, William Brondyk, Bruce L Roberts, Johanne M Kaplan, William M Siders
ABSTRACT

Alemtuzumab, a monoclonal antibody directed against human CD52, is used in the treatment of MS. To characterize the impact of anti-CD52 administration, a monoclonal antibody to mouse CD52 (anti-muCD52) was generated and evaluated in EAE mouse models of MS. A single course of anti-muCD52 provided a therapeutic benefit accompanied by a reduction in the frequency of autoreactive T lymphocytes and production of pro-inflammatory cytokines. Examination of the CNS revealed a decrease in infiltrating lymphocytes, demyelination and axonal loss. Electrophysiological assessment showed preservation of axonal conductance in the spinal cord. These findings suggest that anti-CD52 therapy may help preserve CNS integrity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hematoxylin Solution, Gill No. 1
Sigma-Aldrich
3,3′-Diaminobenzidine, 97%
Sigma-Aldrich
3,3′-Diaminobenzidine, 97% (HPLC)