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HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes

HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Z777169

Proteomix® WCX-NP10 Weak Cation Exchange Column

L × I.D. 5 cm × 4.6 mm, 10 μm particle size, stainless steel

Z777180

Proteomix® WCX-NP1.7 Weak Cation Exchange Column

L × I.D. 5 cm × 4.6 mm, 1.7 μm particle size, stainless steel

Z777185

Proteomix® WCX-NP3 Weak Cation Exchange Column

L × I.D. 5 cm × 4.6 mm, 3 μm particle size, stainless steel

Z777193

Proteomix® WCX-NP5 Weak Cation Exchange Column

L × I.D. 5 cm × 4.6 mm, 5 μm particle size, stainless steel

standard

Product No.
Description
Pricing

L6876

Lysozyme from chicken egg white

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

R6513

Ribonuclease A from bovine pancreas

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

CONDITIONS

column

Proteomix WCX NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777185)

column

Proteomix WCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777180)

column

Proteomix WCX-NP10, 5 cm x 4.6 mm, 10 μm particles (Z777169)

column

Proteomix WCX-NP5, 5 cm x 4.6 mm x 5 μm particles (Z777193)

mobile phase

(A) 20 mM sodium phosphate, pH 6.5, (B) A + 1.0 M NaCl

gradient

0 to 100% B in 25 min, 15 min prewash

flow rate

0.5 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

5 μL

sample

Ribonuclease A, Cytochrome C and Lysozyme (1 mg/mL) min

Description

Legal Information

Proteomix is a registered trademark of Sepax Technologies

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS