HPLC Analysis Proteins on Proteomix® SCX-NP1.7, Demonstration of Column Stability with Repeated Injections
Materials
analytical column
CONDITIONS
column
Proteomix SCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777146)
column
Proteomix SCX-NP1.7 guard cartridge with holder, 1 cm x 4 mm I.D. (Z777144)
mobile phase
(A) 20 mM sodium phosphate buffer, pH 6.5, (B) A + 1.0 M NaCl
gradient
0 - 100% B in 25 min, 15 min prewash
flow rate
0.5 mL/min
column temp.
ambient
detector
UV, 214 nm
injection
5 μL
sample
each compound 1 mg/mL
Description
Legal Information
Proteomix is a registered trademark of Sepax Technologies