Lentivirus Protocols

Successful Transduction Using Lentivirus
Protocol provided by the MISSION^® R&D Team

Safe Handling of Lentivirus
Protocol provided by the MISSION^® R&D Team

CRISPR Lentiviral Screening: Antibiotic Selection
Protocol provided by the MISSION^® R&D Team

Protocol, FACS Titration of Lentivirus
Protocol provided by the MISSION^® R&D Team

Protocol, Screening with SAM CRISPRa Lentiviral Library Technical Bulletin (PDF)
Protocol provided by the MISSION^® R&D Team

Lentiviral Titer by Limiting Dilution Protocol (PDF)
Developed by the MISSION^® RNAi Team

Lentiviral Transduction Protocol (PDF)
Developed by the MISSION^® RNAi Team

Lentiviral Transduction of Suspension Cells—Spinoculation Protocol
Adapted from Dr. Una O'Doherty, University of Pennsylvania School of Medicine

Lentiviral Transduction Protocol for Human Embryonic Stem Cells
Jennifer Moore, Ph.D., Alana Toro-Ramos, M. Sc., and Rick Cohen, Ph.D. Rutgers University Stem Cell Research Center

Determining Relative Transduction of Adherent Cells using MISSION^® TurboGFP™ Transduction Particles
Protocol provided by the MISSION^® R&D Team

Preparation of the Lentiviral Transduction Particles using Lentiviral Packaging Mix
Protocol provided by the MISSION^® RNAi Team

Protocol for Transducing Bone Marrow Derived Macrophages (BMDM) using MISSION^® ExpressMag^® Super Magnetic Kit
Protocol provided by the MISSION^® R&D Team

Optimization of Antibiotic Selection Using a Cytotoxicity Profile
Protocol provided by the MISSION^® R&D Team

Cold Spring Harbor Laboratory Press Protocols

• Culturing Trophoblast Stem (ST) Cell Lines
• In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry
• Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors

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