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739928

Sigma-Aldrich

3-epi-25-Hydroxyvitamin D3 solution

50 μg/mL in ethanol, 98% (CP)

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About This Item

Empirical Formula (Hill Notation):
C27H44O2
CAS Number:
Molecular Weight:
400.64
EC Number:
MDL number:
UNSPSC Code:
12352205
PubChem Substance ID:
NACRES:
NA.24

Quality Level

assay

98% (CP)

form

solution

concentration

50 μg/mL in ethanol

technique(s)

mass spectrometry (MS): suitable

color

colorless

mp

-144.0 °C

application(s)

detection

storage temp.

−20°C

SMILES string

C[C@H](CCCC(C)(C)O)[C@H]1CC[C@H]2\C(CCC[C@]12C)=C\C=C3\C[C@H](O)CCC3=C

InChI

1S/C27H44O2/c1-19-10-13-23(28)18-22(19)12-11-21-9-7-17-27(5)24(14-15-25(21)27)20(2)8-6-16-26(3,4)29/h11-12,20,23-25,28-29H,1,6-10,13-18H2,2-5H3/b21-11+,22-12-/t20-,23-,24-,25+,27-/m1/s1

InChI key

JWUBBDSIWDLEOM-UEGFJKGBSA-N

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General description

3-epi-25-Hydroxyvitamin D3 (C3-epimer) is a metabolite of vitamin D3. It is an epimer of 25-hydroxyvitamin D3 which differs only at C-3 position wherein -OH group at C-3 carbon is in the α orientation rather than in the β orientation.

Application

This product may be used as an analytical standard.

Packaging

1 mL in ampule

pictograms

FlameExclamation mark

signalword

Danger

hcodes

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

wgk_germany

WGK 1

flash_point_f

closed cup

flash_point_c

closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

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Sigma-Aldrich

17937

25-Hydroxyvitamin D2

Overestimation of 25-hydroxyvitamin D3 by increased ionisation efficiency of 3-epi-25-hydroxyvitamin D3 in LC-MS/MS methods not separating both metabolites as determined by an LC-MS/MS method for separate quantification of 25-hydroxyvitamin D3, 3-epi-25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in human serum
van den Ouweland JMW, et al.
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 967, 195-202 (2014)

Articles

The metabolic role and health implications of the various vitamin D isoforms are of current clinical interest. Therefore, it is important to have an analytical method that will resolve all of the known isoforms with necessary sensitivity and specificity

This application shows an Ascentis Express F5 provided rapid resolution of the isobaric vitamin D metabolites. MS detection provides the necessary secondary resolution.

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