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L040030

Sigma-Aldrich

Cap A

configured for ABI

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.21

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

form

liquid

shelf life

365 days

concentration

97.0-119.0 g/L (Acetic Anhydride content, titration)

color

clear colorless, to brownish

compatibility

configured for ABI

storage temp.

2-8°C

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General description

Cap A solution contains acetic anhydrideanhydride, lutidine and tetrahydrofuran (THF). It is used after the phosphoramidite reaction/coupling step. The solution is configured for ABI.

Biochem/physiol Actions

Cap A solution aborts chains bearing unreacted 5′ hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′ hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Tetrahydrofurane / Lutidine / Acetic Anhydride 8/1/1 (v/v/v)

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2

supp_hazards

EUH019

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

6.1 °F

flash_point_c

-14.4 °C

Certificates of Analysis (COA)

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