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L040150

Sigma-Aldrich

Cap 1

Synonym(s):

Cap A

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.21

type

for DNA synthesis

Quality Level

200

product line

Proligo Reagents

form

liquid

storage condition

dry at room temperature

technique(s)

DNA synthesis: suitable

impurities

≤200 ppm water content (Karl Fischer)

color

clear

suitability

conforms to structure (GC, Formulation)

storage temp.

room temp

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General description

Cap 1 solution consists of n-methylimidazole, lutidine, and acetonitrile (ACN).

Application

Cap 1 is suitable for use after the phosphoramidite reaction/coupling step.

Biochem/physiol Actions

Cap 1 solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Acetonitrile / Lutidine / N-Methylimidazole 8/1/1 (v/v/v)

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

35.6 °F - closed cup

flash_point_c

2.0 °C - closed cup

Certificates of Analysis (COA)

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