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H-083

Supelco

25-Hydroxyvitamin D3 solution

100 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

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About This Item

Empirical Formula (Hill Notation):
C27H44O2
CAS Number:
63283-36-3
Molecular Weight:
400.64
EC Number:
200-578-6
MDL number:
MFCD00867077
UNSPSC Code:
41116107
PubChem Substance ID:
329815137
NACRES:
NA.24

grade

certified reference material

Quality Level

300

form

liquid

feature

Snap-N-Spike®/Snap-N-Shoot®

packaging

ampule of 1 mL

manufacturer/tradename

Cerilliant®

concentration

100 μg/mL in ethanol

application(s)

clinical testing
clinical testing
clinical testing

format

single component solution

storage temp.

−70°C

SMILES string

O.C[C@H](CCCC(C)(C)O)[C@H]1CC[C@H]2C(\CCC[C@]12C)=C\C=C3\C[C@@H](O)CCC3=C

InChI

1S/C27H44O2/c1-19-10-13-23(28)18-22(19)12-11-21-9-7-17-27(5)24(14-15-25(21)27)20(2)8-6-16-26(3,4)29/h11-12,20,23-25,28-29H,1,6-10,13-18H2,2-5H3/b21-11+,22-12-/t20-,23+,24-,25+,27-/m1/s1

InChI key

JWUBBDSIWDLEOM-DTOXIADCSA-N

Gene Information

human ... VDR(7421)

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General description

A Certified Spiking Solution® of 25-Hydroxyvitamin D3, a prohormone whose blood serum levels are used to determine Vitamin D nutritional status. This solution standard is suitable for critical quantitative applications in research and clinical environments including: for use in preparation of controls and calibrators for LC/UV and LC-MS/MS assays; as system tuning/suitability standards; linearity verification; internal proficiency testing; and for OEM use in preparation of reagents, development of assays and instrument IQ/OQ. The stable, quantitative solution format provides significant benefits to the user including material savings, increased efficiency of labor, and convenience of use. The shelf stable Snap-N-Spike® format allows laboratories to eliminate painstaking weighing operations and provides the ability to spike a known concentration of analyte into their matrix of choice just before use.

Application

25-Hydroxyvitamin D3 may be used as a certified reference material (CRM) for the determination of the analyte in biological samples by chromatography techniques.

Legal Information

CERILLIANT is a registered trademark of Merck KGaA, Darmstadt, Germany
CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation
Snap-N-Shoot is a registered trademark of Cerilliant Corporation
Snap-N-Spike is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

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Description
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1α,25-Dihydroxyvitamin D3 solution, 5 μg/mL in ethanol, ampule of 1 mL, certified reference material, Cerilliant®

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4-Phenyl-1,2,4-triazoline-3,5-dione, for HPLC derivatization, LiChropur™, ≥98.0% (CHN)

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pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

57.2 °F - closed cup

flash_point_c

14 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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25-Hydroxycholecalciferol ≥98% (HPLC)

Sigma-Aldrich

H4014

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25-Hydroxyvitamin D2 ≥95.0% (HPLC)

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17937

25-Hydroxyvitamin D2

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Sigma-Aldrich

739642

25-Hydroxyvitamin D3 solution

25-Hydroxyvitamin D3 (6,19,19-d3) ≥97 atom % D, ≥98% (CP)

Sigma-Aldrich

705888

25-Hydroxyvitamin D3 (6,19,19-d3)

25-Hydroxyvitamin D3 (6,19,19-d3) solution 50 μg/mL in ethanol, 97 atom % D, 98% (CP)

Sigma-Aldrich

739618

25-Hydroxyvitamin D3 (6,19,19-d3) solution

3-epi-25-Hydroxyvitamin D3 98% (CP)

Sigma-Aldrich

705993

3-epi-25-Hydroxyvitamin D3

New aspects of the plasma carrier protein for 25-hydroxycholecalciferol in vertebrates.
H Van Baelen et al.
Annals of the New York Academy of Sciences, 538, 60-68 (1988-01-01)
J H Soares et al.
Poultry science, 74(12), 1919-1934 (1995-12-01)
Vitamin D is a complex of secosteroids that must undergo metabolic alterations to reach optimal biological activity. The parent compounds 1) ergocalciferol (D2) and 2) cholecalciferol (D3) can be synthesized in the leaves of many plants or in the skin
Antrison Morris et al.
Poultry science, 93(8), 1951-1956 (2014-06-17)
Three experiments were conducted to study the effects of 25-hydroxycholecalciferol supplementation on BW gain, IL-1β, and 1α-hydroxylase mRNA expression in different organs of broiler chickens following a lipopolysaccharide (LPS) injection. In experiment I, birds were fed a basal diet supplemented
A method for simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by using two steps of high-performance liquid chromatography
Okano T, et al.
J. Nutr. Sci. Vitaminol., 27(1), 43-54 (1981)
A Bello et al.
Poultry science, 93(5), 1053-1058 (2014-05-06)
The effects of in ovo injection of 25-hydroxycholecalciferol [25(OH)D3] at various dosages on weight, breaking strength, and ash and mineral concentrations of left tibial bones in Ross × Ross 708 broilers on d 0 and 21 posthatch were investigated. Six
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Ultra high-performance liquid chromatographic (UHPLC) separation of Vitamin D Metabolites― 3-epi-25-hydroxyvitamin D2 and 25-hydroxyvitamin D2, using Supel™ Carbon LC column with baseline separation, excellent peak shape, and sensitivity.

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Chromatograms

application for HPLC

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