03-0188-00
SMC® Human Phospho-α-Synuclein (S129) High Sensitivity Immunoassay Kit
Synonym(s):
p-α-synuclein S129 immunoassay kit, p-α-synuclein Ser129 immunoassay kit, p-α-synuclein Single Molecule Counting Kit, p-α-synuclein ultra-sensitive immunoassay
About This Item
Recommended Products
species reactivity
mouse, monkey, cat, rat
manufacturer/tradename
SMC®
assay range
sensitivity: 0.1 pg/mL
(Lower Limit of Quantification)
inter-assay cv: 7%
intra-assay cv: 6%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days.)
technique(s)
single molecule counting: suitable
storage temp.
2-8°C
Related Categories
General description
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Specificity
Specificity to the following species analytes were tested with the following results:
Monkey – 100%
Cat – 100%
Rat – 100%
Mouse – 25%
Beagle – 25%
Cross Reactivity:
No cross-reactivity was observed to an alpha-synuclein recombinant protein purified from E. Coli (i.e. non-phosphorylated alpha-synuclein).
Accuracy:
CSF Recovery: 5 CSF samples spiked with average recovery of 100% and a range of 95-103%
Serum/Plasma Recovery: 2 serum samples and 3 plasma samples with average recovery of 87% and a range of 83-91%
Application
This new streamline kit has the following features including:
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Removal of a plate transfer step
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
Research Category:
Neuroscience, neurological
Research Sub-Category:
- Neurodegenerative Diseases
- Neuronal & Glial Markers
- Neuroscience
- Nervous System
Packaging
Components
- Assay Buffer
- Phospho-a-synuclein (S129) Coated Beads
- Standard Diluent
- Phospho-a-synuclein (S129) Detection Antibody
- Phospho-a-synuclein (S129) Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
Legal Information
Disclaimer
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
wgk_germany
WGK 3
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Related Content
Discover how to expand neuroscience biomarker research with high sensitivity immunoassays, such as Single Molecule Counting (SMC®) assays. These assays give researchers the tools they need to detect low-abundant blood-based biomarkers and expand biomarker options in neuroscience research.
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