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03-0196-00

Millipore

SMC® Human Total α-synuclein High Sensitivity Immunoassay Kit

Uses 100 µL per well of 1:200 diluted serum or plasma samples, or 1:10 diluted cerebrospinal fluid (CSF) samples.

Synonym(s):

Human α-synuclein, α-synuclein, Human total-α-synuclein, a-synuclein, total-a-synuclein

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

species reactivity

canine, rat, feline, mouse

manufacturer/tradename

SMC®

assay range

sensitivity: 1.95  pg/mL
(LLOQ of Total α-synuclein)
inter-assay cv: 14%
intra-assay cv: 9%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days)

technique(s)

single molecule counting: suitable

Related Categories

General description

The SMC® Human Total-α-Synuclein High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

Specificity

Species Reactivity Note

Specificity to the following species samples were tested with the following results:
Monkey – 100%
Cat – 100%
Rat – 100%
Mouse – 100%
Beagle – 100%

Cross Reactivity

Cross-reactivity to the following analytes were tested with the following results:
Tau – 0%UCHL1 – 0%
β-synuclein – 0%
γ-synuclein – 0%

Accuracy

CSF Recovery: 5 CSF samples spiked with average recovery of 96% and a range of 90-102%
Serum Recovery: 4 serum samples spiked with average recovery of 96% and a range of 93-98%
Plasma Recovery: 4 plasma samples spiked with average recovery of 96% and a range of 93-97%

Application

Uses 100 µL per well of 1:200 diluted serum or plasma samples, or 1:10 diluted cerebrospinal fluid (CSF) samples.

Packaging

96 well assay

Components

  • Assay Buffer
  • Total α-synuclein Coated Beads
  • Standard Diluent
  • Total α-synuclein Detection Antibody
  • Total α-synuclein Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B

Other Notes

  • This new streamline kit has the following features:
  • Improved options for sample prep and incubation
  • Lyophilization protein standard for uniform shipping and storage condition
  • Increased reagent volumes to accommodate automation
  • Able to achieve assay specifications on both SMCxPRO® and Erenna.

Legal Information

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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