06-923
Anti-Cdk1/Cdc2 (PSTAIR) Antibody
Upstate®, from rabbit
Synonym(s):
Anti-CDC2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, amphibian, mouse
species reactivity (predicted by homology)
chicken, canine, starfish, monkey, frog, Drosophila, sheep
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
chicken ... Cdk1(396252)
dog ... Cdk1(100856079)
frog ... Cdk1(394503)
fruit fly ... Cdk1(34411)
human ... CDK1(983)
mouse ... Cdk1(12534)
rat ... Cdk1(54237)
General description
Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Expression of cdc2 plays a critical role in cell transition through the G2/M phase. The activity of cdc2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for cdc2.
Specificity
Cdk1/cdc2 kinase
Wide species cross-reactivity is expected.
Immunogen
Synthetic peptide corresponding to residues 42-57 of human cdc2 kinase. The immunogen is identical in a wide range of species.
Application
Fluorescent Immunocytochemistry Analysis:
A previous lot of this antibody was used in the confocal Immunofluorescent microscopy of A431 cells. Cells were grown, washed, fixed with formaldahyde, and permeabilized with NP40. The cells were triple stained with with Anti-Cdk1/Cdc2 (Red, Cy3), Phalloidin (Actin, Green, AlexaFluor488), and DAPI (Blue, Nucleus). Positive nuclear staining.
Immunoprecipitation: 4 µg of a previous lot immunoprecipitated cdc2 from 500 µg of A431 RIPA lysate.
A previous lot of this antibody was used in the confocal Immunofluorescent microscopy of A431 cells. Cells were grown, washed, fixed with formaldahyde, and permeabilized with NP40. The cells were triple stained with with Anti-Cdk1/Cdc2 (Red, Cy3), Phalloidin (Actin, Green, AlexaFluor488), and DAPI (Blue, Nucleus). Positive nuclear staining.
Immunoprecipitation: 4 µg of a previous lot immunoprecipitated cdc2 from 500 µg of A431 RIPA lysate.
Anti-Cdk1/Cdc2 (PSTAIR) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Cdk1/Cdc2 (PSTAIR) & has been validated in ICC, IP & WB.
Quality
Evaluated by western blot on human A431, mouse 3T3 and/or rat L6 RIPA cell lysates.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected cdc2 from human A431, mouse 3T3 or rat L6 RIPA cell lysates.
Western Blot Analysis: 0.5-2 µg/mL of this antibody detected cdc2 from human A431, mouse 3T3 or rat L6 RIPA cell lysates.
Target description
~34 kDa
Physical form
Format: Purified
Purified rabbit serum in Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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flash_point_f
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flash_point_c
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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M G Lee et al.
Nature, 327(6117), 31-35 (1987-05-07)
A human homologue of the cdc2 gene has been cloned by expressing a human cDNA library in fission yeast and selecting for clones that can complement a mutant of cdc2. The predicted protein sequence of the human homologue is very
Up-regulation of 14-3-3zeta in lung cancer and its implication as prognostic and therapeutic target.
Tao Fan et al.
Cancer research, 67(16), 7901-7906 (2007-08-19)
A functional genomic approach integrating microarray and proteomic analyses done in our laboratory has identified 14-3-3zeta as a putative oncogene whose activation was common and driven by its genomic amplification in lung adenocarcinomas. 14-3-3zeta is believed to function in cell
Platelet-activating factor- and thrombin-induced stimulation of p34cdc2-cyclin histone H1 kinase activity in platelets.
Samiei, M, et al.
The Journal of Biological Chemistry, 266, 14889-14892 (1991)
Quantification of cyclin B1 and p34(cdc2) in bovine cumulus-oocyte complexes and expression mapping of genes involved in the cell cycle by complementary DNA macroarrays.
Claude Robert, Isabelle Hue, Serge McGraw, Dominic Gagne, Marc-Andre Sirard
Biology of reproduction null
Tsvetomira Ivanova et al.
Nature communications, 11(1), 2267-2267 (2020-05-10)
To faithfully transmit genetic information, cells must replicate their entire genome before division. This is thought to be ensured by the temporal separation of replication and chromosome segregation. Here we show that in 20-40% of unperturbed yeast cells, DNA synthesis
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