Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody

The kidneys play a major role in the regulation of blood glucose. Although kidney nephrons indiscriminatly filter about 180g of glucose per day from the blood, most is reabsorbed actively in the proximal convoluted tubule by a Na+/Gl co-transport (ratio 2:1) mechanism (SGLT). Typically, glucose is first reabsorbed by these transporters, which reside within the brush-border epithelia of the PCT cells. The glucose is then transported out of the cell across the basolateral membranes by a facilitated diffusion sugar transporter (GLUTs). At least three members of SGLT′s (SGLT1-3) have been cloned and characterized from various species. SGLT1-3 genes code for protein of approximately 659-672 residues, with both N and C termini predicted to be extracellular. There is approximately 60-70% homology between the 3 forms. SGLT1 does not discriminate a-MDG, glucose and galactose, while SGLT2 and 3 do not transport D-galactose efficiently. SGLT1 is an integral membrane protein expressed in proximal convoluted tubule Sq1 segments of the kidney (outer renal medulla) and in the intestine.

Recognizes SGLT-1.

Synthetic peptide corresponding to amino acids 402-420 in the cytoplasmic region of SGLT-1 of mouse/rabbit small intestine.

Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody detects level of SGLT-1 (Sodium Glucose Co-transporter-1) & has been published & validated for use in WB, IH(P), ELISA.

Immunohistochemisty (paraffin): Anti-SGLT-1 staining on human kidney, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was used at 1 μg/mL, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the brush boarder of proximal convoluted tubule.

ELISA: 1:10,000 - 1:50,000 dilution of a previous lot, using 50-100 ng of control peptide/well.

Optimal working dilutions must be determined by end user.

Western Blot: A previous lot of this antibody was used on multiple lysates. See datasheet.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Routinely evaluated by Western Blot on PC3 lysates.

Western Blot Analysis:
1:500-1:2,000 dilution of this lot detected SGLT-1 on 10 μg of PC3 lysates.

Recognizes a band of approximately 70-77 kDa in Western blot.

Replaces: AB1352

Rabbit polyclonal IgG antiserum in buffer containing 0.1% sodium azide.

Stable for 1 year at -20°C from date of receipt in undiluted aliquots.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
PC3 cell lysate

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