07-1496
Anti-CaM Kinase II Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Anti-CAMKA, Anti-CaMKIINalpha, Anti-CaMKIIalpha, Anti-MRD53, Anti-MRT63
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, chicken, bovine, hamster, human, rabbit, rat, guinea pig
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CAMK2A(815)
General description
The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.
Specificity
Recognizes proteins of 50-72 kDa, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II α subunits expressed in sf9 insect cells are also detected. As the sequences of the rat α, δ, and γ isoforms are conserved over this amino terminus region, this antibody is expected to recognize the δ, γ, and α isoforms. Antibody is not expected to cross-react with the β isoform. Proteins of unknown identity may be detected on immunoblot analysis with some lysates (40, 45 and 90 kDa).
Immunogen
Epitope: N-terminus
Synthetic peptide corresponding to the N-terminus of Rat CaMK II
Application
Anti-CaM Kinase II Antibody is an antibody against CaM Kinase II for use in WB.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
Quality
Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500 to 1:1,000 dilution of this lot detected CaM Kinase II on 10 μg of HEK293 lysates.
Western Blot Analysis:
1:500 to 1:1,000 dilution of this lot detected CaM Kinase II on 10 μg of HEK293 lysates.
Target description
50-72 kDa, corresponding to apparent molecular mass of CaMKII isoforms.
Linkage
Replaces: AB3111
Physical form
Affinity purified
Purified rabbit polyclonal IgG in buffer containing borate buffered saline and phosphate buffered saline containing 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C in undiluted aliquots from date of receipt. For optimal storage, aliquot and store at -20°C or -70°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HEK293 cell lysate
HEK293 cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Find documentation for the products that you have recently purchased in the Document Library.
C Rongo et al.
Nature, 402(6758), 195-199 (2000-01-26)
Synaptic connections undergo a dynamic process of stabilization or elimination during development, and this process is thought to be critical in memory and learning and in establishing the specificity of synaptic connections. The type II calcium- and calmodulin-dependent protein kinase
R M Tombes et al.
Biochimica et biophysica acta, 1452(1), 1-11 (1999-10-19)
Over two dozen alternative splice variants of CaMK-II, the type II Ca(2+)/CaM-dependent protein kinase, are encoded from four genes (alpha, beta, gamma and delta) in mammalian cells. Isozymes of alpha and beta CaMK-II are well characterized in brain; however, an
F Gardoni et al.
FEBS letters, 456(3), 394-398 (1999-08-26)
Ca2+/calmodulin-dependent protein kinase II (CaMKII), a multifunctional, widely distributed enzyme, is enriched in post-synaptic densities (PSDs). Here, we demonstrate that CaMKII binds to a discrete C-terminal region of the NR2A subunit of NMDA receptors and promotes the phosphorylation of a
Y H Koh et al.
Cell, 98(3), 353-363 (1999-08-24)
Discs large (DLG) mediates the clustering of synaptic molecules. Here we demonstrate that synaptic localization of DLG itself is regulated by CaMKII. We show that DLG and CaMKII colocalize at synapses and exist in the same protein complex. Constitutively activated
Anand M Prasad et al.
Vascular pharmacology, 87, 172-179 (2016-09-24)
Angiotensin-II (Ang-II) is a well-established mediator of vascular remodeling. The multifunctional calcium-calmodulin-dependent kinase II (CaMKII) is activated by Ang-II and regulates Erk1/2 and Akt-dependent signaling in cultured smooth muscle cells in vitro. Its role in Ang-II-dependent vascular remodeling in vivo
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