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Sigma-Aldrich

Nitro blue tetrazolium chloride (NBT)

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About This Item

CAS Number:
298-83-9
Molecular Weight:
817.64
MDL number:
MFCD00012159
UNSPSC Code:
41116130
NACRES:
NA.21

form

solid

mp

189 °C

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

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General description

Nitro blue tetrazolium chloride (NBT) is a dry dye for the staining of histological tissue sections and histochemical as well as in vivo methods on material of human origin. The dye has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining.

Application

for microscopy

Analysis Note

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (Methanol)256 - 259 nmSpec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; methanol; calc. on dried substance)800 - 900Loss on drying (Vacuum < 7 mbar, P₂O₅, 24 h)≤ 12 %Suitability for microscopypasses test

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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