Skip to Content
MilliporeSigma
All Photos(1)

Documents

1.24823

Sigma-Aldrich

Nitro blue tetrazolium chloride (NBT)

for microscopy

Synonym(s):

Nitro blue tetrazolium chloride (NBT), 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis [2-(4-nitrophenyl)-5-phe nyl-2H-tetrazolium chloride], NBT

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
298-83-9
Molecular Weight:
817.64
MDL number:
MFCD00012159
UNSPSC Code:
41116124
EC Index Number:
206-067-4

Quality Level

400

form

solid

mp

189 °C

εmax

800-900 at 256-259 nm in methanol at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Nitro blue tetrazolium chloride (NBT) is a dry dye for the staining of histological tissue sections and histochemical as well as in vivo methods on material of human origin.
The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

Dry dye used in histochemistry

Analysis Note

Identity (UV/VIS-Spectrum)passes testAbsorption maximum λ max (Methanol)256 - 259 nmSpec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; methanol; calc. on dried substance)800 - 900Loss on drying (Vacuum < 7 mbar, P₂O₅, 24 h)≤ 12 %Suitability for microscopypasses test

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 5

1 of 5

NBT/BCIP Stock Solution

Roche

11681451001

NBT/BCIP Stock Solution

Phenazine methosulfate &#8805;90% (UV)

Sigma-Aldrich

P9625

Phenazine methosulfate

NBT-BCIP&#174; solution BioReagent, suitable as substrate for alkaline phosphatase in dot blots

Sigma-Aldrich

72091

NBT-BCIP® solution

Sigma-Aldrich

Sigma-Aldrich

A4108

Aminophenyl fluorescein solution

BCIP solution, &gt;95% (HPLC), pkg of 3&#160;mL (150 mg)

Roche

11383221001

BCIP

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service