189724
Ascorbate Oxidase, Cucurbita sp.
Native ascorbate oxidase from Cucurbita species. Catalyzes the oxidation of ascorbioxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid.
Synonym(s):
Ascorbate Oxidase, Cucurbita sp.
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About This Item
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Quality Level
form
lyophilized
specific activity
≥1500 U/mg
≥300 units/mg material
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
shipped in
ambient
storage temp.
−20°C
General description
Native ascorbate oxidase from Cucurbita species. A multi-copper-containing oxidase that catalyzes oxidation of ascorbic acid to dehydroascorbic acid. AO catalyses the four-electron reduction of dioxygen to water with concominant one-electron oxidation of the reducing organic substrate. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid. Inhibited by azide, dithiocarbamate, and thiocyanate.
Native ascorbate oxidase from Cucurbita species. Catalyzes oxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid. Inhibited by azide, dithiocarbamate, and thiocyanate.
Caution
enzyme will denature below pH 4.0.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will oxidize 1.0 µmol ascorbic acid per min at 37°C, pH 5.6.
Reconstitution
Following reconstitution refrigerate (4°C). This Stock solutions are stable for up to 3 weeks at 4°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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