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Sigma-Aldrich

Cholesterol Oxidase, Streptomyces sp.

Cholesterol Oxidase, Streptomyces sp. CAS 9028-76-6, is a native cholesterol oxidase that catalyzes the oxidation of cholesterol to cholesterone and hydrogen peroxide.

Synonym(s):

Cholesterol Oxidase, Streptomyces sp., Cholesterol:oxygen oxidoreductase

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54

Quality Level

form

lyophilized solid

specific activity

≥3 units/mg solid
≥40 U/mg

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

yellow

solubility

water: 20 mg/mL

foreign activity

Catalase ≤1%
glucose oxidase and uricase ≤0.01%

shipped in

ambient

storage temp.

−20°C

General description

Native cholesterol oxidase from Streptomyces species. Catalyzes oxidation of cholesterol to cholesterone and hydrogen peroxide (isoelectric points: 6.1 and 7.3). Suitable for determination of total cholesterol when coupled with cholinesterase and peroxidase. Has an optimal pH of 6.0-8.0.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will oxidize 1.0 µmol of cholesterol per min at 25°C, pH 7.0.

Other Notes

Gadda, G., et al. 1997. Eur. J. Biochem. 250, 369.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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