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Cleland′s Reagent, Molecular Biology Grade

A protective agent for SH groups.

Synonym(s):

Cleland’s Reagent, Molecular Biology Grade, 1,4-Dithiothreitol, DTT

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About This Item

Empirical Formula (Hill Notation):
C4H10O2S2
CAS Number:
Molecular Weight:
154.25
MDL number:
UNSPSC Code:
12161504

grade

Molecular Biology

Quality Level

assay

≥99% (titration)

form

powder
solid

reaction suitability

reagent type: reductant

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

water: 15 mg/mL

cation traces

heavy metals: ≤0.001%

absorption

≤0.100 at 280 nm at 100 mM (1 cm)
≤0.500 at 260 nm at 100 mM (1 cm)

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1

InChI key

VHJLVAABSRFDPM-IMJSIDKUSA-N

General description

A protective agent for SH groups.
Water-soluble protective reagent for sulfhydryl groups [absorbance (100 mM): ≤0.5 at 260 nm; ≤0.1 at 280 nm]. It is capable of maintaining monothiols completely in the reduced state and of reducing disulfides quantitatively. Since it readily permeates cell membranes, it can protect protein sulfhydryls and restore enzyme activity lost by oxidation.

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

CorrosionExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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DL-Dithiothreitol ≥99.0% (RT)

Sigma-Aldrich

43819

DL-Dithiothreitol

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