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444235-M

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MMP-13 Substrate, Fluorogenic

A quenched fluorescence substrate for MMP-13 (collagenase-3; kcat/Km = 1.09 x 10⁶ M⁻¹s⁻¹).

Synonym(s):

MMP-13 Substrate, Fluorogenic, Collagenase-3 Substrate, Fluorogenic

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About This Item

Empirical Formula (Hill Notation):
C51H65N13O15
Molecular Weight:
1100.14
UNSPSC Code:
12352200

Quality Level

100

assay

≥98% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

yellow

solubility

DMSO: 5 mg/mL

fluorescence

λex ~325 nm
λem ~393 nm

storage temp.

−20°C

General description

A quenched fluorescence substrate for MMP-13 (collagenase-3; kcat/Km = 1.09 x 106 M-1s-1). Less efficient as a substrate for MMP-1 (fibroblast collagenase) or MMP-8 (neutrophil collagenase).
A quenched fluorescence substrate for MMP-13 (collagenase-3; kcat/Km = 1.09 x 106 M-1s-1; excitation maximum: ~325 nm; emission maximum: ~393 nm). Less efficient as a substrate for MMP-1 (fibroblast collagenase) or MMP-8 (neutrophil collagenase).

Biochem/physiol Actions

Primary Target
A quenched fluorescence substrate for MMP-13 (collagenase-3)
kcat/Km = 1.09 x 106 M⁻¹ sec⁻¹ for MMP-13 (collagenase-3)

Warning

Toxicity: Standard Handling (A)

Sequence

MCA-Pro-Cha-Gly-Nva-His-Ala-Dpa-NH₂ [Cha = L-cyclohexylalanine; Dpa = 3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl; Nva = L-norvaline]

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Knauper, V., et al. 1996. J. Biol. Chem. 271, 1544.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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