444282
MMP/TACE Substrate, FS-6
The widely used MMP substrate FS-1 is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities.
Synonym(s):
MMP/TACE Substrate, FS-6, Mca-KPLG~L-Dpa-AR-NH₂
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About This Item
Empirical Formula (Hill Notation):
C55H80N16O16
Molecular Weight:
1221.32
UNSPSC Code:
12352200
Recommended Products
Quality Level
assay
≥97% (HPLC)
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
protect from light
solubility
water: 20 mg/mL
shipped in
ambient
storage temp.
2-8°C
General description
The widely used MMP substrate FS-1 (Cat. No. 03-32-5032) is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities. Exhibits 2- to 9-fold higher specificity constant (kcat/Km) towards MMP-1, -8, -13, -14 when compared with FS-1 and ~2-fold higher kcat/Km towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10.
The widely used MMP substrate FS-1 (Cat. No. 03-32-5032), modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and sensitivity. Exhibits a 2- to 9-fold higher specificity constant (kcat/Km) towards MMP-1, -8, -13, -14 (Km (μM)= 27.5, 5.2, and 7.9, respectively) when compared with FS-1 and ~ 2-fold higher kcat/Km (778, 400 M-1 s-1) towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10. Excitation max: 324 nm; Emission max: 400 nm.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Broad-spectrum MMP and TACE substrate
Broad-spectrum MMP and TACE substrate
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Warning
Toxicity: Standard Handling (A)
Sequence
Mca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH₂
Physical form
Supplied as a trifluoroacetate salt.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Neumann, U., et al. 2004. Anal. Biochem.328, 166.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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