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70527

Sigma-Aldrich

100 mM IPTG Solution - Novagen

Convenient, ready-to-use solution

Synonym(s):

100 mM IPTG Solution - Novagen, IPTG Solution, 100 mM, Isopropyl -β-D-1-thiogalactopyranoside, Isopropylthio -β-D-galactoside

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About This Item

CAS Number:
367-93-1
MDL number:
MFCD00063273
UNSPSC Code:
12352200

Quality Level

100

form

solution

mol wt

238.30  g/mol

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−20°C

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

Related Categories

Application

100 mM IPTG Solution - Novagen has been used to:

  • induce expression of an anti-CD11b-nanobody with a sortase recognition tag in Chalmers (WK6) cells
  • induce expression in calcium-independent sortase from BL21(DE3) cells
  • turn on bacterial protein production of the protein A/G-MNase

Biochem/physiol Actions

Isopropyl-β-D-1-thiogalactopyranoside (IPTG) is an inducer of the lacoperon. It is highly recommended for blue/white screening using lacZα-complementation in appropriate vectors and host strains, for example, pSTBlue-1/NovaBlue. In addition, it is also used for inducing protein expression with the pET system and various lac promoter-derived expression systems. IPTG is supplied as a sterile aqueous solution and is free of dioxane. It comes in convenient volumes of 10 × 1.5 ml. IPTG is used in bacterial cultures to induce the expression of plasmid-based genes to produce recombinant proteins under the control of the lac promoter.

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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