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BugBuster® HT Protein Extraction Reagent

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About This Item

UNSPSC Code:
41106511

form

liquid

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

BugBuster HT Protein Extraction Reagent combines BugBuster Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Application

BugBuster® HT Protein Extraction Reagent has been used:
  • to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
  • for protein extraction from bacterial cell pellet
  • used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein

Features and Benefits

  • Fast protein extraction and nucleic acid degradation
  • Ideal for processing various samples of different volumes
  • Increased extraction is ensured by the addition of rLysozyme solution

Warning

Toxicity: Standard Handling (A)

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

Sigma-Aldrich

B7435

CelLytic™ B Cell Lysis Reagent

Benzonase® Nuclease, ultrapure ≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade

Sigma-Aldrich

E8263

Benzonase® Nuclease, ultrapure

Lysozyme from chicken egg white lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Sigma-Aldrich

L6876

Lysozyme from chicken egg white

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Articles

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® Plus Lysonase™ Kit: Cell Lysis and Nucleic Acid Removal for Gram-negative and Gram-positive Bacteria

Cell lysis and nucleic acid removal for Gram-negative and Gram-positive bacteria using the BugBuster Plus Lysonase™ Kit

Use of BugBuster® and Lysonase™ Reagents for efficient protein extraction from Gram-positive bacteria

The combination of BugBuster and Lysonase greatly enhances the release of soluble proteins from Gram-positive bacteria.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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