71077
X-Gal Solution - Novagen
Convenience for blue/white screening
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About This Item
UNSPSC Code:
12352200
Recommended Products
Quality Level
form
solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
tissue culture: suitable
shipped in
wet ice
storage temp.
−20°C
General description
X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing. X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.
Application
X-Gal Solution - Novagen has been used at 1:40 dilution to detect the expression of β-galactosidase and mutant fusion protein generated by the ApcΔ242 mutation in the tissue sample.
Warning
Toxicity: Irritant (B)
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
188.6 °F - (refers to pure substance)
flash_point_c
87 °C - (refers to pure substance)
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