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AB1529

Sigma-Aldrich

Anti-Nitric Oxide Synthase I Antibody

serum, Chemicon®

Synonym(s):

NOS I, bNOS, nNOS

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

sheep

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rabbit, rat, guinea pig

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NM_000620.1

UniProt accession no.

P29475

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

guinea pig ... Nos1(100729682)
rabbit ... Nos1(100009243)
rat ... Nos1(24598)

Specificity

bNOS (neuronal). Not yet tested for cross-reactivity to iNOS or eNOS. See reference below.

Immunogen

A 21 amino acid peptide (1409-1429) of the neuronal form of NOS from rat cerebellum, coupled to KLH. The sequence is H-RSESIAFIEESKKDADEVFSS-NH2.

Application

Detect Nitric Oxide Synthase I using this Anti-Nitric Oxide Synthase I Antibody validated for use in WB, IH.
Immunohistochemistry: 1:500-1,000

Immunoblotting

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress

Physical form

Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.

Storage and Stability

Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Direct projections from substance P-containing neurons to nitric oxide synthase-containing interneurons in the rat striatum.
Li, Jin Lian, et al.
Neuroscience Letters, 318, 133-136 (2002)
Postnatal downregulation of inhibitory neuromuscular transmission to the longitudinal muscle of the guinea pig ileum.
Bian X, Burda JE, Carrasquillo M, Galligan JJ
Neurogastroenterology and Motility null
Localization of muscarinic receptors M1R, M2R and M3R in the human colon.
A M Harrington,C J Peck,L Liu,E Burcher,J M Hutson,B R Southwell
Neurogastroenterology and Motility null
Nils Wierup et al.
BMC neuroscience, 8, 51-51 (2007-07-13)
The peptide CART is widely expressed in central and peripheral neurons, as well as in endocrine cells. Known peripheral sites of expression include the gastrointestinal (GI) tract, the pancreas, and the adrenal glands. In rodent pancreas CART is expressed both
CART-peptide immunoreactivity in enteric nerves in patients with Hirschsprung's disease.
A Gunnarsdottir,N Wierup,L T Larsson,M J Kuhar,E Ekblad
European Journal of Pediatric Surgery : Official Journal of Austrian Association of Pediatric Surgery ... [Et Al] = Zeitschrift fur Kinderchirurgie null
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