AB15568
Anti-Aquaporin 7 Antibody
Chemicon®, from rabbit
Synonym(s):
AQP7
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About This Item
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... AQP7(364)
Specificity
Recognizes Aquaporin 7 (AQP7).
Immunogen
Purified peptide from the N-terminus of rat AQP7 (Accession number P56403).
Application
Anti-Aquaporin 7 Antibody detects level of Aquaporin 7 & has been published & validated for use in IC, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western blot: 1:200-1:600 using ECL on rat and mouse kidney membrane lysate.
Immunohistochemistry on rat testis sections.
Immunocytochemistry: 1:800 using 3T3-L1 adipocytes.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on rat testis sections.
Immunocytochemistry: 1:800 using 3T3-L1 adipocytes.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions must be determined by the end user.
Physical form
Format: Purified
ImmunoAffinity Purified
Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Storage and Stability
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
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Fertility and sterility, 95(4), 1514-1517 (2010-12-07)
The aquaglyceroporin aquaporin 7 (AQP7) is dynamically expressed in mouse uteri undergoing decidualization after implantation. The expansion of AQP7 during uterine decidualization is associated with elevated uterine glycerol accumulation and glycerol kinase expression, suggesting that glycerol might be a potential
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