Anti-Integrin beta1 Antibody, Cytosolic

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Reactive with human, mouse and rat integrin β-1, as confirmed by western blot. Not reactive with other integrins.

Epitope: C-terminus

Synthetic peptide corresponding to the c-terminal cytoplasmic domain of human β-1 integrin.

Anti-Integrin beta1 Antibody, Cytosolic detects level of Integrin beta1 & has been published & validated for use in IP & WB.

Immunoprecipitation:
1 μL of antiserum per 25 μg total cell protein (in 40 μL). ) 0.5% triton X-100 lysis buffer with proteinase inhibitors is suggested.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Western Blot Analysis:
1:500 to 1:1000 dilution of this antibody was used for detection of integrin beta1 in whole cell lysate under non-reducing. Size of beta 1 integrin under non-reduced conditions is approximately 110 kDa; often a 220 kDa dimer is observed as well; beta -1 under reduced conditions runs as a 130kDa band with higher molecular weight aggregates as well.

~130 kDa

Rabbit polyclonal serum containing no preservatives

Unpurified

Stable for 1 year at -20ºC from date of receipt.

Control
U251 cell line

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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