Anti-Neurofilament M (145 kDa) Antibody, CT

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.

Strong and specific staining for NF-M (145 kDa)

Epitope: C-terminus

Recombinant fusion protein containing the C-terminal 168 amino acids of rat NF-M

Anti-Neurofilament M (145 kDa) Antibody, C-terminus detects level of Neurofilament M (145 kDa) & has been published & validated for use in IC, IH, IH(P) & WB.

Immunohistochemistry:
A 1:200 dilution of a previous lot was used in Immunohistochemistry.
Staining is not affected by neurofilament phosphorylation. Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum

Immunohistochemistry(paraffin):

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot: 1:1000 dilution of this lot detected Neurofilament M on 10 μg of mouse brain lysates..

145 kDa

Neat rabbit antiserum containing no preservative.

Unpurified

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Rat enteric neurons, rat spinal cord tissue Brain, Peripheral Nerve

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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