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M4403

Sigma-Aldrich

Monoclonal Anti-MAP2 antibody produced in mouse

clone HM-2, ascites fluid

Synonym(s):

MAP2 Antibody - Monoclonal Anti-MAP2 antibody produced in mouse, Map2 Antibody

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About This Item

MDL number:
MFCD00164683
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HM-2, monoclonal

mol wt

antigen ~280 kDa

contains

15 mM sodium azide

species reactivity

rat, chicken, human, mouse, bovine, quail

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: 1:1,000 using a fresh total rat brain extract or an enriched microtubule protein preparation

isotype

IgG1

UniProt accession no.

P11137

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

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General description

Microtubule-associated protein 2 (MAP2) is a neuronal protein. It belongs to the MAP2/Tau family. This gene is located to human chromosome 2q34-q35. MAP2 is present in neurons. MAP2 is the major microtubule-associated protein of brain tissue. There are three forms of MAP2; two are similarly sized with apparent molecular weights of 280kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70kDa (MAP2c).

Specificity

Reacts with all known forms of MAP2, namely MAP2a, MAP2b and MAP2c. Does not cross-react with other MAPs or tubulin. By immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue, the antibody shows selective labeling of dendritic trees throughout the brain.

Immunogen

rat brain microtubule-associated proteins (MAPs).

Application

Monoclonal Anti-MAP2 (2a+2b) antibody has been used in immunohistochemistry, immunostaining and western blotting.

Biochem/physiol Actions

Microtubule-associated protein 2 (MAP2) interacts with microtubules to maintain the structure of dendrites. It plays a major role in supporting the actin cytoskeleton in spines, binding and nucleating filamentous actin (f-actin) to modulate spine morphology.

Physical form

Supplied as ascites fluid with 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

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wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Neural differentiation of mouse embryonic stem cells grown in monolayer.
Pachernik J, et al.
Reproduction, Nutrition, Development, 42(4), 317-326 (2002)
GABA and histogenesis in fetal and neonatal mouse brain lacking both the isoforms of glutamic acid decarboxylase.
Ji F, et al.
Neuroscience Research, 33(3), 187-194 (1999)
An optimal antigen retrieval method suitable for different antibodies on human brain tissue stored for several years in formaldehyde fixative.
Evers P and Uylings HBM
Journal of Neuroscience Methods, 72(2), 197-207 (1997)
Loss of microtubule-associated protein 2 immunoreactivity linked to dendritic spine loss in schizophrenia.
Shelton MA, et al.
Biological Psychiatry, 78(6), 374-385 (2015)
The MAP2/Tau family of microtubule-associated proteins.
Dehmelt L and Halpain S
Genome Biology, 6(1), 204-204 (2005)
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