AB6000
Anti-TIMP-3 Antibody, CT
from rabbit, purified by affinity chromatography
Synonym(s):
MIG-5, TIMP metallopeptidase inhibitor 3, Tissue inhibitor of metalloproteinases 3
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
horse, human, rat
species reactivity (predicted by homology)
pig (based on 100% sequence homology), primate (based on 100% sequence homology), equine (based on 100% sequence homology), canine (based on 100% sequence homology), monkey (based on 100% sequence homology), mouse (95% homology), bovine (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TIMP3(7078)
General description
Tissue inhibitor of metalloproteinases 3 (TIMP-3) is a member of the TIMP family. The TIMPs are so named because they are slow, tight binding endogenous inhibitors of the Matrix Metalloproteinases (MMPs). The four TIMPs have different inhibition constants for the different MMPs studied. TIMPs have been shown to have activity against the ADAMs family of proteinases. TIMP-3 is an efficient "sheddase" inhibitor, inhibiting ADAM-17 (TACE) at the low nanomolar levels seen with MMP inhibition. The other ADAMs proteinases have not yet been assayed for TIMP inhibition, but TIMP-2 seems to be much less active on ADAM-17 than isTIMP-3. TIMP-3 is thought to be constitutively produced by many cell types and is inducible in others. The TIMP-3 localization differs from the other 3 TIMPs, and is thought to be primarily deposited into the extracellular matrix.
Specificity
The antibody recognizes TIMP-3 at the C-terminus. Does not appear to cross react with TIMP-1 or TIMP-2.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to human TIMP-3 at the C-terminus.
Application
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected TIMP-3 in C6 cells.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
This Anti-TIMP-3 Antibody, C-terminus is validated for use in WB, IC for the detection of TIMP-3.
Quality
Evaluated by Western Blot using an HL-60 cell lysate supplemented with PMA-conditioned media.
Western Blot Analysis: 0.5 µg/ml of this antibody detected TIMP-3 on 10 µg of HL-60 in PMA-conditioned media.
Western Blot Analysis: 0.5 µg/ml of this antibody detected TIMP-3 on 10 µg of HL-60 in PMA-conditioned media.
Target description
Bands may be observed for reduced protein bands at ~ 24 kDa (unglycosylated) and ~ 30 kDa (glycosylated). Additional TIMP-3 bands MAY be visible at 50 kDa (dimer), 12 kDa, and 15 kDa (breakdown products) and sample dependent.
Linkage
Replaces: AB802
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HL-60 cell lysate in PMA-conditioned media.
HL-60 cell lysate in PMA-conditioned media.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ioannis Kanakis et al.
Arthritis & rheumatology (Hoboken, N.J.), 71(4), 571-582 (2018-11-01)
Cartilage destruction in osteoarthritis (OA) is mediated mainly by matrix metalloproteinases (MMPs) and ADAMTS. The therapeutic candidature of targeting aggrecanases has not yet been defined in joints in which spontaneous OA arises from genetic susceptibility, as in the case of
Qitao Zhang et al.
Experimental eye research, 178, 212-222 (2018-10-20)
The daily shedding and renewal of photoreceptor outer segments (OS) is critical for maintaining vision. This process relies on the efficient uptake, degradation, and sorting of shed OS material by the retinal pigment epithelium (RPE). Poor OS degradation has been
Jung Hyun Park et al.
Journal of personalized medicine, 12(5) (2022-05-29)
Tissue inhibitor of metalloproteinase-3 (TIMP-3) is a component of the extracellular environment and is suggested to play an indirect role in regulating Aβ production and the pathophysiology of Aβ deposition in brains. However, studies on the amount of TIMP-3 in
Sarah Naessens et al.
Human mutation, 40(5), 539-551 (2019-01-23)
Sorsby fundus dystrophy (SFD) is a macular degeneration caused by mutations in TIMP3, the majority of which introduce a novel cysteine. However, the exact molecular mechanisms underlying SFD remain unknown. We aimed to provide novel insights into the functional consequences
Anna Paola Carreca et al.
International journal of molecular sciences, 22(5) (2021-03-07)
Ectodomain shedding is a key mechanism of several biological processes, including cell-communication. Disintegrin and metalloproteinases (ADAMs), together with the membrane-type matrix metalloproteinases, play a pivotal role in shedding transmembrane proteins. Aberrant shedding is associated to several pathological conditions, including arthritis.
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