ABE1044
Anti-HSF 1 Antibody
serum, from rabbit
Synonym(s):
Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
technique(s)
ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HSF1(3297)
General description
HSF1, also known as Heat shock factor protein 1 (HSF-1) or Heat shock transcription factor 1 (HSTF-1), and encoded by the gene HSF1/HSTF1, is a DNA binding transcription factor that binds heat shock promoter elements (HSE’s) and activates transcription. In higher eukaryotes, HSF1 is unable to bind to HSE regions unless the cells are heat shocked. HSF1 is normally a monomer that interacts with HSP90 and other chaperones in a large complex in the cytoplasm. Upon heat shock HSF1 is released from the complex, dimerizes, and is transported into the nucleus. HSF1 is localized to the cytoplasm during normal growth the protein localizes to the nucleus upon heat shock. HSF1 is part of the cellular stress response, and HSF1 is known to interact with many other stress- induced proteins including NCOA6, SYMPK, RALBP1, HSF2, CEBPB, HSPA1A, Heat shock protein 90kDa alpha and HSPA.
Immunogen
Recombinant protein corresponding to mouse HSF 1.
Application
Anti-HSF 1 antibody is an antibody against HSF 1 for use in western blotting, IP & ChIP.
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from HEK293 cell lysates transfected with wild type or mutant hRP1-HA (Fujimoto, M., et al. (2012). Mol Cell. 48(2):182-194.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from hHSF1 transfected MEF cell lysate (Fujimoto, M., et al. (2010). Mol Cell Biol. 21(1):106-116.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from wild type and HSF2-null primary MEF cell lysates (Shinkawa, T., et al. (2011). Mol Cell Biol. 22(19):3571-3583.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from HeLa and MEF cell lysates which were either untreated or heat shock treated (Prof. A. Nakai, Yamaguchi University School of Medicine).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from hHSF1 transfected MEF cell lysate (Fujimoto, M., et al. (2010). Mol Cell Biol. 21(1):106-116.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from wild type and HSF2-null primary MEF cell lysates (Shinkawa, T., et al. (2011). Mol Cell Biol. 22(19):3571-3583.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated HSF 1 from HeLa and MEF cell lysates which were either untreated or heat shock treated (Prof. A. Nakai, Yamaguchi University School of Medicine).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting: A 1:1,000 dilution of this antibody detected HSF 1 in 10 µg of HeLa cell lysate.
Western Blotting: A 1:1,000 dilution of this antibody detected HSF 1 in 10 µg of HeLa cell lysate.
Target description
~75 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Toyohide Shinkawa et al.
Molecular biology of the cell, 22(19), 3571-3583 (2011-08-05)
Heat shock response is characterized by the induction of heat shock proteins (HSPs), which facilitate protein folding, and non-HSP proteins with diverse functions, including protein degradation, and is regulated by heat shock factors (HSFs). HSF1 is a master regulator of
Arpit Katiyar et al.
FEBS open bio, 10(6), 1135-1148 (2020-04-18)
The mitochondrial unfolded protein response (UPRmt ) is characterized by the transcriptional induction of mitochondrial chaperone and protease genes in response to impaired mitochondrial proteostasis and is regulated by ATF5 and CHOP in mammalian cells. However, the detailed mechanisms underlying
Ke Tan et al.
Nature communications, 6, 6580-6580 (2015-03-13)
Heat-shock response is an adaptive response to proteotoxic stresses including heat shock, and is regulated by heat-shock factor 1 (HSF1) in mammals. Proteotoxic stresses challenge all subcellular compartments including the mitochondria. Therefore, there must be close connections between mitochondrial signals
Ryosuke Takii et al.
The EMBO journal, 38(24), e102566-e102566 (2019-10-28)
The recruitment of RNA polymerase II (Pol II) to core promoters is highly regulated during rapid induction of genes. In response to heat shock, heat shock transcription factor 1 (HSF1) is activated and occupies heat shock gene promoters. Promoter-bound HSF1
Mitsuaki Fujimoto et al.
Molecular cell, 48(2), 182-194 (2012-09-04)
Transcription factor access to regulatory elements is prevented by the nucleosome. Heat shock factor 1 (HSF1) is a winged helix transcription factor that plays roles in control and stressed conditions by gaining access to target elements, but mechanisms of HSF1
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