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ABN445

Sigma-Aldrich

Anti-ARL13B Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

ARL13B, ARL2-like protein 1, ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

NP_878899

UniProt accession no.

Q3SXY8

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ARL13B(200894)

General description

ARL13B/ARL2L1 is an important neuronal developmental protein, as well as, a cilium-specific protein required for the control of the microtubule-based ciliary axoneme structure. ARL13B/ARL2L1 is required for proper patterning in neural development during neural tube formation, and ARL13B/ARL2L1 is critical for cerebral cortex development and the initial polarization of radial glia scaffold, the first step in cortex development. ARL13B/ARL2L1 also controls the migration and placement of post mitotic interneurons. ARL13B/ARL2L1 is found in cellular projections and cilium and is widely expressed in the developing system as well in the gut. ARL13B/ARL2L1 also is a member of the small GTPase superfamily as it binds GTP but does not cleave it.

Immunogen

KLH-conjugated linear peptide corresponding to human ARL13B.

Application

Anti-ARL13B Antibody is an antibody against ARL13B for use in Immunohistochemistry and Immunocytochemistry.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ARL13B in human cerebellum, embryonic mouse liver, and human cerebral cortex tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in mouse E13-14 brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected ARL13B in 10 µg of mouse E13-14 brain tissue lysate.

Target description

~60 kDa observed. Western blot shows the absence of a 60 kDa Arl13b band in Arl13bE9.5 (Humbert, M.C., et al. (2012). PNAS. 109(48):19691-19696).

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Melissa C Humbert et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(48), 19691-19696 (2012-11-15)
Mutations affecting ciliary components cause a series of related genetic disorders in humans, including nephronophthisis (NPHP), Joubert syndrome (JBTS), Meckel-Gruber syndrome (MKS), and Bardet-Biedl syndrome (BBS), which are collectively termed "ciliopathies." Recent protein-protein interaction studies combined with genetic analyses revealed

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