AC111S
Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy5 Conjugate
from sheep, CY5 conjugate
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46
Recommended Products
biological source
sheep
Quality Level
conjugate
CY5 conjugate
antibody form
purified antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
mouse
technique(s)
immunocytochemistry: suitable
shipped in
wet ice
target post-translational modification
unmodified
Related Categories
General description
Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.
Specificity
Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.
Immunogen
Purified mouse IgG.
Application
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Fragment Specific Secondary Antibodies
Sheep Anti-Mouse IgG Antibody, Species absorbed, Cy5 Conjugate is an antibody against Sheep Mouse IgG for use in Immunocytochemistry.
Quality
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: Performance of this Cy5-conjugated secondary antibody has been validated by indirect immunofluorescent labelling (1:250 dilution) of A431 cells following a mouse monoclonal anti-H3K27me3 primary antibody (Cat. No. 05-1951).
Immunocytochemistry Analysis: Performance of this Cy5-conjugated secondary antibody has been validated by indirect immunofluorescent labelling (1:250 dilution) of A431 cells following a mouse monoclonal anti-H3K27me3 primary antibody (Cat. No. 05-1951).
Target description
~150 kDa observed.
Physical form
Fractionated by octanoic acid and ammonium sulfate cut, and then depleted with rat IgG column and mouse IgA column prior to Cy3 conjugation.
Purified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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