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ECM104

Sigma-Aldrich

Millicoat® Human Collagen Type I Coated Strips (96-Wells)

96-well plate coated with human Collagen Type I used for cell adhesion studies.

Synonym(s):

Formerly under the CytoMatrix ™ brand name.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32161000
NACRES:
NA.75

Quality Level

100

species reactivity

human

manufacturer/tradename

Chemicon®
Millicoat®

technique(s)

activity assay: suitable
cell based assay: suitable

input

sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
sample type pancreatic stem cell(s)

NCBI accession no.

NM_000088.3

UniProt accession no.

P02452

detection method

colorimetric

shipped in

wet ice

Gene Information

human ... COL1A1(1277)

Related Categories

General description

Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion and migration of many diverse cell types on various extracellular matrix (ECM) component proteins. Millicoat Cell Adhesion Strips are provided as 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The wells in rows A - G have been coated with Human Collagen Type I. Row H of each strip is coated with BSA which serves as a negative assay control. Cells are seeded onto the coated substrate. Subsequently, adherent cells are fixed and stained. Relative attachment is determined using absorbance readings.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLICOAT is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Articles

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocols

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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