ECM Cell Adhesion Array Kit, fluorometric

Introduction:

Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion, migration, and invasion of many diverse cell types on various extracellular matrix (ECM) component proteins. The CHEMICON^® ECM Cell Adhesion Array Kit (Fluorometric) provides an ECM Array microtiter plate with a homogenous fluorescence detection format, allowing for large-scale screening and quantitative comparison of multiple samples/cell lines. The kit is designed as a rapid (1-2 hours), cost effective, efficient method for characterization of cell adhesion.

In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

· Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530)

· Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531)

· Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532)

· Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM533)

· Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorometric) (ECM534)

· Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Fluorometric) (ECM535)

· ECM Cell Adhesion Array Kit (Colorimetric) (ECM540)

· QCM 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)

· QCM 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)

· QCM 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)

· QCM 96-well Cell Invasion Assay (ECM555)

· QCM 96-well Collagen-Based Cell Invasion Assay (ECM 556)

· 24-well Insert Cell Migration and Invasion assay systems

· CytoMatrix Cell Adhesion strips (ECM protein coated)

· QuantiMatrix ECM protein ELISA kits



Test Principle:

The CHEMICON ECM Cell Adhesion Array kit utilizes a homogenous fluorescence detection format, allowing large-scale screening and quantitative comparison of multiple samples. Each kit contains a 96-well, microtiter plate consisting of 12 X 8-well removable strips. Each well within a strip (7 wells total) is pre-coated with a different ECM protein (see table below) along with one BSA-coated well (negative control). Experimental cells are seeded onto the coated substrates, where adherent cells are captured. Subsequently, unbound cells are washed away, and the adherent cells are lysed and detected by the patented CyQuant GR dye (Molecular Probes) (1-2). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (3). Relative cell attachment is determined using a fluorescence plate reader.

Additional information about the ECM components provided in the kit:

Research Category
Cell Structure

The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.

The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.

For Research Use Only. Not for use in diagnostic procedures.

96 wells

ECM Array Plate: (PN: 90652) One 96-well plate with 12 strips. Each 8-well strip consists of 7 different human, ECM protein-coated wells (Collagen I, Collagen II, Collagen IV, Fibronectin, Laminin, Tenascin, Vitronectin) and one BSA-coated well (negative control). See the plate layout on data sheet.

4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.

CyQuant GR^™ Dye: (Part No. 90132) One vial - 75 μL.

Assay Buffer: (Part No. 90601) One bottle - 100 mL.

The ECM Array Plate can be stored at 2° to 8°C in the foil pouch up to its expiration date. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. Keep the remaining kit components at 2° to 8°C.

Precautions:

No data is available on the biological toxicity of CyQuant GRâ dye. This reagent binds to nucleic acids, and as such, should be treated as a potential mutagen which may cause cancer and heritable genetic damage. Handle with caution. The DMSO stock solution should be handled with special caution as DMSO can facilitate the entry of organic molecules into tissues.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

GR is a trademark of Sigma-Aldrich Co. LLC

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.