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FCMAB184F

Sigma-Aldrich

Milli-Mark® Anti-CD19 -FITC Antibody, clone HD37

clone HD37, Milli-Mark®, from mouse

Synonym(s):

Differentiation antigen CD19

B-lymphocyte surface antigen B4

T-cell surface antigen Leu-12, B-lymphocyte antigen CD19

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44

biological source

mouse

Quality Level

100

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HD37, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1κ

NCBI accession no.

NP_001761.3

UniProt accession no.

P15391

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD19(930)

Related Categories

General description

CD19 is a 120 kDa transmembrane glycoprotein with a reduced MW of 95 kDa (Pezzutto, 1987; Sato, 1997). CD19 is a member of the immunoglobulin superfamily with two extracellular C2-type domains (Pezzutto, 1987), and it is a critical signal transduction molecule that regulates B lymphocyte development, activation, and differentiation (Sato, 1997). CD19 expression is restricted to B cells, being absent from T cells, monocytes, and granulocytes (Pezzutto, 1987). The CD19 antigen appears early during B cell maturation, probably at late pro-B cell stage around the time of Ig heavy chain rearrangement. It then persists during all stages of B cell maturation and is lost upon terminal differentiation to plasma cells (Pezzutto, 1987).

Specificity

Antibody recognizes Human CD19.

Immunogen

Hairy cell leukemia cells

Application

Detect CD19 using this Milli-Mark Anti-CD19 -FITC Antibody, clone HD37 validated for use in FC.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by flow cytometry using Human Lymphocytes

Target description

120kDa (Pezzutto, 1987; Sato, 1997)

Physical form

Protein A purified
Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2

Storage and Stability

Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.

Analysis Note

Control
Human Lymphocytes

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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