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MAB10404

Sigma-Aldrich

Anti-Ghrelin Antibody, clone 1ML-1D7

ascites fluid, clone 1ML-1D7, from mouse

Synonym(s):

ghrelin/obestatin prepropeptide1, ghrelin/obestatin2, ghrelin, growth hormone secretagogue receptor ligand1, obestatin1, ghrelin/obestatin preprohormone, Motilin-related peptide2, ghrelin1, M46 protein3, Growth hormone secretagogue

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1ML-1D7, monoclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_057446.1

UniProt accession no.

Q9UBU3

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GHRL(51738)

General description

Ghrelin is a 28 amino acids peptide with an essential n-octonayl modification at Serine 3. This peptide hormone is known as an endogenous ligand for the growth hormone secretagogue receptor and shares sequence similarity with the peptide hormone Motilin. Ghrelin displays pleotropic actions including growth hormone secretion, feeding behaviour and gastrointestinal motility. It is predominantly produced by enteroendocrine cells from the gastrointestinal tract, but has been shown to play a significant role in neurotrophy, particularly in the hippocampus, and appears to be essential for cognitive adaptation to changing environments and the process of learning.

Specificity

This antibody recognizes Ghrelin at the N-terminus.

Immunogen

Linear Peptide corresponding to the N-terminus of human Ghrelin.

Application

Detect Ghrelin using this Anti-Ghrelin Antibody, clone 1ML-1D7 validated for use in WB & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Ghrelin in A431 cells.

Quality

Evaluated by Western Blot in mouse stomach tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Ghrelin in 10 µg of mouse stomach tissue lysate.

Target description

13 kDa calculated and observed

Analysis Note

Control
Mouse stomach tissue lysate

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Histopathologic and immunohistochemical sequelae of bariatric embolization in a porcine model.
Paxton, BE; Alley, CL; Crow, JH; Burchette, J; Weiss, CR; Kraitchman, DL; Arepally, A; Kim, CY
Journal of Vascular and Interventional Radiology : Jvir null

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