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MAB4369

Sigma-Aldrich

Anti-SRF [Serum Response Factor] Antibody, clone 1E1

clone 1E1, from mouse

Synonym(s):

serum response factor (c-fos serum response element-binding transcription factor)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

1E1, monoclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

input

sample type neural stem cell(s)
sample type mesenchymal stem cell(s)

isotype

IgG1κ

UniProt accession no.

P11831

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ELK3(2004)

Related Categories

General description

Serum response factor (SRF) is a nuclear transcription factor that binds to the serum response element (SRE), and is essential for embryonic development of the mesoderm, as well as for the maintenance of adult muscle cells and neurons. As this protein is involved in the downstream regulation of many immediate-early genes, it participates in a variety of cell functions, including cell cycle, apoptosis, cell growth, and cell differentiation. SRF is most commonly activated by serum, LPA, LPS, TPA, growth factors, including TNFalpha, as well as antioxidants and UV light.

Specificity

A weak interaction with mouse has also been observed. Reactivity with other species has not been tested.
This monoclonal antibody recognizes SRF.

Immunogen

Synthetic human SRF peptide, a.a. 406-508.

Application

Detect SRF [Serum Response Factor] using this Anti-SRF [Serum Response Factor] Antibody, clone 1E1 validated for use in WB & IC.
Immunocytochemistry: Recommended working dilution is 1:500.
Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells

Neural Stem Cells

Quality

(Based upon representative data from a previous lot).

Western Blotting: Recommended working dilution is 0.5 µg/mL.

Target description

~52 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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