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MAB5384A4

Sigma-Aldrich

Anti-NG2 Antibody, clone 132.39, Alexa Fluor™488 Conjugate

clone 132.39, from mouse, ALEXA FLUOR™ 488

Synonym(s):

Chondroitin sulfate proteoglycan 4, Chondroitin sulfate proteoglycan NG2, HSN tumor-specific antigen

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

132.39, monoclonal

species reactivity

rat

species reactivity (predicted by homology)

mouse, human

technique(s)

immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

NP_112284

UniProt accession no.

Q00657

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CSPG4(1464)
mouse ... Cspg4(121021)
rat ... Cspg4(81651)

General description

NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.

Specificity

This antibody recognizes the native, recombinant, and protein from cells which express NG2.

Immunogen

Cell line expressing a truncated form of NG2

Application

Anti-NG2 Antibody, clone 132.39, Alexa Fluor™488 Conjugate detects level of NG2 & has been published & validated for use in ICC.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.

Target description

The unconjugated parent antibody (Cat. No. MAB5384) has an observed molecular weight at ~280 kDa

Physical form

Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Alexa Fluor™ 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Rat oligodendrocyte precursor cells

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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