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MABC1035

Sigma-Aldrich

Anti-Gremlin-1 Antibody, clone 2H1.1

clone 2H1.1, from mouse

Synonym(s):

Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, BMP antagonist 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, Increased in high glucose protein 2, IHG-2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2H1.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_037504

UniProt accession no.

O60565

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... GREM1(26585)

General description

Gremlin-1 (UniProt: O60565; also known as Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, BMP antagonist 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, Increased in high glucose protein 2, IHG-2) is encoded by the GREM1 (also known as CKTSF1B1, DAND2, DRM, PIG2) gene (Gene ID: 26585 ) in human. Gremlin-1 is a homodimeric cytokine that plays an important role during carcinogenesis and metanephric kidney organogenesis. Two isoforms of Gremlin-1 have been described that are produced by alternative splicing. Gremlin-1 acts as an antagonist of BMP2 and inhibits BMP2-mediated differentiation of osteoblasts. It also down-regulates the BMP4 signaling in a dose-dependent manner. Gremlin-1 is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off. Gremlin-1 is highly expressed in small intestine where its expression is restricted to intestinal subepithelial myofibroblasts (ISEMFs) at the crypt base. It is also expressed in fetal brain and colon and weak expression is observed in adult brain ovary, prostate, pancreas, and skeletal muscle. Its levels are down-regulated in tumor cell lines. Mutations in GREM1 gene are known to cause Polyposis syndrome, mixed hereditary 1 (HMPS1) that is characterized by apparent autosomal dominant inheritance of multiple types of colorectal polyp, with colorectal carcinoma occurring in a high proportion of affected individuals.

Specificity

Clone 2H1.1 detects Gremlin-1 in human cells. It targets an epitope within 89 amino acids from the internal region of isoform 1.

Immunogen

GST-tagged recombinant fragment corresponding to 89 amino acids from the internal region.

Application

Anti-Gremlin-1, clone 2H1.1, Cat. No. MABC1035, is a mouse monoclonal antibody that detects Gremlin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Gremlin-1 in human prostate and human colon tissues.

Quality

Evaluated by Western Blotting in Gremlin recombinant protein.

Western Blotting Analysis: 1 µg/mL of this antibody detected Gremlin-1 recombinant protein.

Target description

~36 kDa observed; 20.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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