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MABE126

Sigma-Aldrich

Anti-SR protein family Antibody, clone 16H3

clone 16H3, from mouse

Synonym(s):

splicing factor, arginine/serine-rich 6, arginine/serine-rich splicing factor 6, splicing factor, arginine/serine-rich, 55 kDa, Pre-mRNA-splicing factor SRP55, pre-mRNA splicing factor SRP55

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

16H3, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_006266

UniProt accession no.

Q13247

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SRSF6(6431)

General description

Ser-Arg-rich (SR) proteins make up a family of functionally and structurally conserved phosphoproteins, and are required components of alternative and constitutive pre-mRNA splicing. SR proteins are characterized by their modular composition consisting of two domains of interest: an N-terminal RNA recognition motif (RRM) which serves in the determination of DNA-binding specificity, and the RS domain, an arginine and serine rich, C-terminal domain that serves as a shuttling and localization director of SR proteins and as a splicing activation domain. They are involved in various aspects of pre-mRNA splicing and in spliceosome assembly including the identification and appropriation of splice-sites and the manipulation of alternative splicing regulation.

Specificity

This antibody recognizes the four nuclear proteins SRp75, SRp55, SRp40, and SRp20, plus ~20 other prominent nuclear proteins believed to correspond to the SR family.

Immunogen

Dephosphorylated bovine SRp55 and GST-tagged recombinant dephosphorylated SRp55 (Neugebauer, KM et. al 1995).

Application

Anti-SR protein family Antibody, clone 16H3 is a Mouse Monoclonal Antibody for detection of SR protein family also known as arginine/serine-rich splicing factor 6 & has been validated in WB, IP, ICC.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP (Neugebauer, KM, et al. (1995).

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Neugebauer, KM, et al. (1995).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

Quality

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.5 µg/mL of this antibody detected SR proteins in 10 µg of HeLa nuclear extract.

Target description

Multiple SR protein family members detected. Reported to predominantly detect SRp75, SRp55, SRp40, SRp30, SRp20 (Neugebauer, KM, et al. (1995).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa nuclear extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tyler Funnell et al.
Nature communications, 8(1), 7-7 (2017-02-25)
CDC-like kinase phosphorylation of serine/arginine-rich proteins is central to RNA splicing reactions. Yet, the genomic network of CDC-like kinase-dependent RNA processing events remains poorly defined. Here, we explore the connectivity of genomic CDC-like kinase splicing functions by applying graduated, short-exposure
Antonella Pinto et al.
British journal of pharmacology, 172(11), 2918-2927 (2015-01-28)
Dehydroepiandrosterone (DHEA) is thought to be an anti-glucocorticoid hormone known to be fully functional in young people but deficient in aged humans. Our previous data suggest that DHEA not only counteracts the effect of cortisol on RACK1 expression, a protein

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